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Details
(DEVD)₂-R110, non-fluorescent peptidyl (DEVD)-Rhodamine 110-bisamide, is a highly sensitive, photostable fluorogenic substrate for the determination of caspase-3 activity. Characteristic for substrates based on Rhodamine 110 (R110) is that they contain two identical amino acids or peptides attached to a single fluorophor thereby suppressing its visible absorption and its fluorescence. Upon enzymatic cleavage, this substrate is converted first to the fluorescent monoamide and then to the even more fluorescent R110. The spectral properties of these hydrolysis products are similar to those of fluorescein, with peak excitation and emission wavelengths of 496 and 520 nm, respectively. The fluorescence intensity can be measured by flow cytometry, microwell plate reader, or fluorescence microscopy. Useful for high throughput screening applications.
Catalog Number
M-2620
APC Number
81-6-67
Synonyms
(Asp-Glu-Val-Asp)2-Rhodamine 110, (DEVD)₂-R110
Molecular Formula
C₅₆H₆₆N₁₀O₂₃
Relative Molecular Mass
1247.19
CAS Registry Number
220846-75-3
Source
Synthetic
Storage Conditions
-20 ± 5 °C
Keywords
Caspase-3 substrate
Application
Apoptosis & Necrosis
Links / Documents:
Bachem product citations
4049432
Enzyme | Substrates | Inhibitors |
---|---|---|
"Caspase-3 (CPP-32; Yama; Apopain)" |