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Details
The fluorogenic HIV-1 protease substrate DABCYL-Gaba-SQNYPIVQ-EDANS consists of an octapeptide with a fluorescent donor (EDANS) and a quenching acceptor (DABCYL), attached at the COOH- and NH₂-termini. The γ-Abu spacer was inserted to avoid potential steric hindrance of substrate binding by the bulky acceptor. This FRET substrate is cleaved by the HIV-1 protease at the Tyr-Pro bond which results in a time-dependent increase in fluorescence intensity; excitation at 340 nm, emission at 490 nm.
Catalog Number
M-1865
APC Number
72-3-30
One Letter Code
DABCYL-Gaba-SQNYPIVQ-EDANS
Molecular Formula
C₇₃H₉₇N₁₇O₁₈S
Relative Molecular Mass
1532.74
CAS Registry Number
[127134-13-8] net
Source
Synthetic
Storage Conditions
-20 ± 5 °C
Keywords
HIV protease FRET substrate
Areas of Interest
Infectious Disease
Links / Documents:
Brochures:
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Bachem product citations
Product citations
M.W.Pennington et al.,
Peptides 1992, Proceedings of the 22nd European Peptide Symposium, Interlaken, Switzerland, p. 936, C.H.Schneider and A.N.Eberle, eds., Escom, Leiden,
(1993)
4030716
| Enzyme | Substrates | Inhibitors |
|---|---|---|
| Retropepsin | ||
| HIV-2 Protease |
