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Details
Boc-VPR-AMC, a specific, highly fluorogenic substrate for thrombin and the thrombin-staphylocoagulase complex. The thrombin assay has been used for the indirect determination of coagulation factor III. Trypsin, tryptase from rat mast cells, and two trypsin-like enzymes from sperm of the ascidian Halocynthia roretzi, acrosin and spermosin, also hydrolyze this substrate. Furthermore, Boc-VPR-AMC is cleaved by the Kex2 endoprotease (kexin, previously known as proteinase yscF), a membrane-bound, calcium-dependent serine protease from yeast α-cells. Kinetic data for Thrombin: kcat = 105, Km = 21 uM.
Catalog Number
I-1120
Molecular Formula
C₃₁H₄₅N₇O₇
Relative Molecular Mass
627.74
CAS Registry Number
65147-04-8
Source
Synthetic
Storage Conditions
-20 ± 5 °C
Application
Hematology
Links / Documents:
Bachem product citations
J.van Soom et al.,
MedChemComm,
6,
1954 (2015)
Product citations
T.Morita et al.,
Meth. Enzymol.,
80,
311 (1981)
4003460
Enzyme | Substrates | Inhibitors |
---|---|---|
Kexin | ||
Thrombin (Coagulation Factor IIa) | ||
Trypsin | ||
Tryptase |