This internally quenched fluorogenic peptide substrate contains anthranilic acid as fluorescent donor and m-nitro-tyrosine as acceptor (quencher). Its sequence is based on the sequence of hemagglutinin. The FRET substrate is efficiently cleaved by furin, a subtilisin-like eukaryotic serine endoprotease with Km = 3.8 µM and kcat = 29.3 s-1 (kcat/Km = 7'710'000 M-1s-1). Its kcat/Km value is over 2000-fold higher than that of the commonly used substrate Boc-Arg-Val-Arg-Arg-AMC (I-1645).
FRET Substrate for the basic residue-specific yeast aspartyl protease yapsin 1.
M-2115
Abz-Arg-Val-Lys-Arg-Gly-Leu-Ala-m-nitro-Tyr-Asp-OH
Trifluoroacetate salt
| C54H84N18O16 | Mr: 1241.37 | [174838-79-0] net |
| Longterm storage temperature: | -20 ± 5 °C | C54H84N18O16 |
Literature:
H.Angliker et al., Anal. Biochem., 224, 409 (1995)
N.X.Cawley et al., J. Biol. Chem., 278, 5523 (2003)
H.Angliker et al., Anal. Biochem., 224, 409 (1995)
N.X.Cawley et al., J. Biol. Chem., 278, 5523 (2003)
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